http://phys.org/news/2016-01-high-fidel ... tions.html" onclick="window.open(this.href);return false;
TL;DR: Off-target DNA breaks have been by far the most significant limitation of CRISPR-Cas9 genome engineering so far, being the strongest argument for refraining from its use in human application and gene drives. It was the largest limitation when human embryos were genetically modified by CRISP-Cas9 the first time a year ago. Now the limitation is gone.
So now, essentially, it's possible to quickly, efficiently, and cheaply edit, modify, activate, silence, destroy, repair, and even entirely replace with something else any gene sequence we want, anytime we want, anywhere we want, now with practically no unwanted side-effects that we know of.
Pretty difficult to overstate how excited I am about this, and where it will be in two years, or five years, or ten.
The paper:
http://www.nature.com/nature/journal/va ... 16526.html" onclick="window.open(this.href);return false;
Cool science thing, CRISPR again
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